Review



cd45 (til) microbeads, mouse  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Miltenyi Biotec cd45 (til) microbeads, mouse
    Cd45 (Til) Microbeads, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+(TIL)+MicroBeads%2C+mouse/custom%40130-110-618%4042476286
    Average 96 stars, based on 134 article reviews
    cd45 (til) microbeads, mouse - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    FACS:

    Article Title: PROK2 defines a pathogenic neutrophil subset and serves as a clinical indicator of neutrophilic inflammation in COPD.
    Article Snippet: .. CD45+ immune cells were enriched via magnetic-activated cell sorting using mouse CD45 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Article Title: Methods and compositions for treatment of pancreatic cancer
    Article Snippet: .. Tumor-infiltrating leukocytes were positively selected by magnetically activated cell sorting using mouse CD45 MicroBeads (Miltenyi Biotec). ..

    Incubation:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: After digestion, the cell suspensions were passed through a 70μm cell strainer and washed twice in MACS buffer (PBS+0.5% BSA+2mM EDTA) and incubated for 5 minutes on ice with TruStain FcXTM (anti-mouse CD16/32, Biolegend #101320, 10μg/ml). .. Afterwards, cells were incubated with mouse CD45 microbeads (Miltenyi, 130-052-301, 1:10 final concentration), and anti-mouse CD45.2AlexaFluor488. .. After 10 minutes incubation at 4°C, TotalSeq-A hashtag antibodies (Biolegend, see table for CITE-seq panels) were added as follows: hashtag 1 to 5 controls; hashtags 6 to 10: NK-depleted.

    Article Title: Erythroferrone derived from osteoblasts regulates stress erythropoiesis.
    Article Snippet: Author contributions: P.N.-P., G.v.C., T.Y., M.Z., and Y.Z.G. designed research; P.N.-P., G.v.C., M.P., E.T., A.G., R.W., E.I., F.S., F.K., and M.L. performed research; L.K., T.Y., M.Z., and Y.Z.G. contributed new reagents/analytic tools; P.N.-P., G.v.C., M.P., E.T., A.G., R.W., E.I., F.S., F.K., and M.L. analyzed data; and P.N.-P., T.Y., M.Z., Y.Z.G. wrote the paper; and all authors reviewed and edited the paper.

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction
    Article Snippet: After digestion, the cell suspensions were passed through a 70 μm cell strainer and washed twice in MACS buffer (PBS + 0.5% BSA + 2 mM EDTA) and incubated for 5 min on ice with TruStain FcXTM (anti-mouse CD16/32, Biolegend #101320, 10 μg/ml). .. Afterwards, cells were incubated with mouse CD45 microbeads (Miltenyi, 130-052-301, 1:10 final concentration), and anti-mouse CD45.2-AlexaFluor488. .. After 10 min incubation at 4 °C, TotalSeq-A hashtag antibodies (Biolegend, see table for CITE-seq panels) were added as follows: hashtag 1 to 5 controls; hashtags 6 to 10: NK-depleted.

    Concentration Assay:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: After digestion, the cell suspensions were passed through a 70μm cell strainer and washed twice in MACS buffer (PBS+0.5% BSA+2mM EDTA) and incubated for 5 minutes on ice with TruStain FcXTM (anti-mouse CD16/32, Biolegend #101320, 10μg/ml). .. Afterwards, cells were incubated with mouse CD45 microbeads (Miltenyi, 130-052-301, 1:10 final concentration), and anti-mouse CD45.2AlexaFluor488. .. After 10 minutes incubation at 4°C, TotalSeq-A hashtag antibodies (Biolegend, see table for CITE-seq panels) were added as follows: hashtag 1 to 5 controls; hashtags 6 to 10: NK-depleted.

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction
    Article Snippet: After digestion, the cell suspensions were passed through a 70 μm cell strainer and washed twice in MACS buffer (PBS + 0.5% BSA + 2 mM EDTA) and incubated for 5 min on ice with TruStain FcXTM (anti-mouse CD16/32, Biolegend #101320, 10 μg/ml). .. Afterwards, cells were incubated with mouse CD45 microbeads (Miltenyi, 130-052-301, 1:10 final concentration), and anti-mouse CD45.2-AlexaFluor488. .. After 10 min incubation at 4 °C, TotalSeq-A hashtag antibodies (Biolegend, see table for CITE-seq panels) were added as follows: hashtag 1 to 5 controls; hashtags 6 to 10: NK-depleted.

    Magnetic Cell Separation:

    Article Title: γδ T cell-stromal networks modulate matrix composition and vascularity in foreign body response.
    Article Snippet: Dead AR TI CL E IN P RE SS cells were removed with Magnetic-Activated Cell Sorting (MACS) using the Dead Cell Removal kit (Miltenyi Biotec 130-090-101). .. MACS with the mouse CD45 MicroBeads (Miltenyi Biotec 130-052-301) was used to separate and collect the CD45+ immune cells (positive fraction) and CD45- stromal cells (negative fraction). ..

    Staining:

    Article Title: Durable control of autoimmunity requires sustained stimulation of regulatory T cells.
    Article Snippet: For intracellular staining, cells were fixed and permeabilized with the eBioscience Foxp3 Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Cat. #00-5523-00) per manufacturer’s instructions. .. For intracellular cytokine staining, CD45+ leukocytes were enriched from pancreatic tissue preparations using mouse CD45 MicroBeads (Miltenyi Biotec, Cat. #130-052-301) according to the manufacturer’s protocol. ..

    other:

    Article Title: Proteasome alteration between epithelial and hematopoietic cells facilitates positive selection of CD8 T cells.
    Article Snippet: AR TI CL E IN P RE SS Flow cytometric analysis was performed on FACS LSR II or FACS Fortessa (BD Biosciences).



    Similar Products

    96
    Miltenyi Biotec cd45 (til) microbeads, mouse
    Cd45 (Til) Microbeads, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+(TIL)+MicroBeads%2C+mouse/custom%40130-110-618%4042476286
    Average 96 stars, based on 1 article reviews
    cd45 (til) microbeads, mouse - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec mouse miltenyi
    Mouse Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+(TIL)+MicroBeads%2C+mouse/pm42468528-262-33-34
    Average 96 stars, based on 1 article reviews
    mouse miltenyi - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec automacs pro separator
    Automacs Pro Separator, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+MicroBeads%2C+mouse/pmc13425456-305-12-15
    Average 97 stars, based on 1 article reviews
    automacs pro separator - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd45 magnetic beads
    Cd45 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+MicroBeads%2C+mouse/pm42462537-203-34-37
    Average 97 stars, based on 1 article reviews
    cd45 magnetic beads - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd45 population 230
    Cd45 Population 230, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+MicroBeads%2C+mouse/pm42393387-138-35-38
    Average 97 stars, based on 1 article reviews
    cd45 population 230 - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd45 cells
    Cd45 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+MicroBeads%2C+mouse/pm42392076-1039-4-14
    Average 97 stars, based on 1 article reviews
    cd45 cells - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd45 positive cells
    (A) Representative images (left panel) and quantification (right panel) of p16 + cancer cells in treatment-naive (n=17) and gemcitabine/cisplatin (Gem/Cis)-treated (n=22) human CCA. (B) Telomere-associated foci (γH2AX immunofluorescence with telomere fluorescence in situ hybridization) (left panel) and quantification (right panel) in p16 + cancer cells. (C-E) Sequential immunofluorescence quantifying p16 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells <t>(CD45</t> + ), and the proliferative status (Ki67) of p16 + cells. (F) Senescence-associated β-galactosidase activity in human RBE CCA cells, proliferating versus doxorubicin or Gem/Cis-induced. (G) Single-nucleus RNA sequencing of human CCA (n=10 patients): UMAP of cell populations and expression of CDKN2A , CDKN1A , and MKI67 with senescence (SenMayo, Fridman, and Reactome cellular senescence) gene-set scores. (H) Multivariable Cox regression of senescence transcriptional scores and overall survival, adjusted for age, sex, and stage, performed independently in the Mayo discovery cohort (n=158, 108 events) and an external Asian cohort (OEP00105, n=244, 99 events). White arrows, senescent-like cancer cells. Scale bar, 50 µm. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.
    Cd45 Positive Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+(TIL)+MicroBeads%2C+mouse/bio_rxiv__64898__2026__06__24__734341-213-0-10
    Average 96 stars, based on 1 article reviews
    cd45 positive cells - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd45 183 microbeads
    (A) Representative images (left panel) and quantification (right panel) of p16 + cancer cells in treatment-naive (n=17) and gemcitabine/cisplatin (Gem/Cis)-treated (n=22) human CCA. (B) Telomere-associated foci (γH2AX immunofluorescence with telomere fluorescence in situ hybridization) (left panel) and quantification (right panel) in p16 + cancer cells. (C-E) Sequential immunofluorescence quantifying p16 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells <t>(CD45</t> + ), and the proliferative status (Ki67) of p16 + cells. (F) Senescence-associated β-galactosidase activity in human RBE CCA cells, proliferating versus doxorubicin or Gem/Cis-induced. (G) Single-nucleus RNA sequencing of human CCA (n=10 patients): UMAP of cell populations and expression of CDKN2A , CDKN1A , and MKI67 with senescence (SenMayo, Fridman, and Reactome cellular senescence) gene-set scores. (H) Multivariable Cox regression of senescence transcriptional scores and overall survival, adjusted for age, sex, and stage, performed independently in the Mayo discovery cohort (n=158, 108 events) and an external Asian cohort (OEP00105, n=244, 99 events). White arrows, senescent-like cancer cells. Scale bar, 50 µm. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.
    Cd45 183 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+MicroBeads%2C+mouse/10__1158_slash_1535___7163__mct___25___0349-104-5-8
    Average 97 stars, based on 1 article reviews
    cd45 183 microbeads - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd45 microbeads
    (A) Representative images (left panel) and quantification (right panel) of p16 + cancer cells in treatment-naive (n=17) and gemcitabine/cisplatin (Gem/Cis)-treated (n=22) human CCA. (B) Telomere-associated foci (γH2AX immunofluorescence with telomere fluorescence in situ hybridization) (left panel) and quantification (right panel) in p16 + cancer cells. (C-E) Sequential immunofluorescence quantifying p16 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells <t>(CD45</t> + ), and the proliferative status (Ki67) of p16 + cells. (F) Senescence-associated β-galactosidase activity in human RBE CCA cells, proliferating versus doxorubicin or Gem/Cis-induced. (G) Single-nucleus RNA sequencing of human CCA (n=10 patients): UMAP of cell populations and expression of CDKN2A , CDKN1A , and MKI67 with senescence (SenMayo, Fridman, and Reactome cellular senescence) gene-set scores. (H) Multivariable Cox regression of senescence transcriptional scores and overall survival, adjusted for age, sex, and stage, performed independently in the Mayo discovery cohort (n=158, 108 events) and an external Asian cohort (OEP00105, n=244, 99 events). White arrows, senescent-like cancer cells. Scale bar, 50 µm. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.
    Cd45 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd45+microbeads/CD45+(TIL)+MicroBeads%2C+mouse/pm42331382-413-29-33
    Average 96 stars, based on 1 article reviews
    cd45 microbeads - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    (A) Representative images (left panel) and quantification (right panel) of p16 + cancer cells in treatment-naive (n=17) and gemcitabine/cisplatin (Gem/Cis)-treated (n=22) human CCA. (B) Telomere-associated foci (γH2AX immunofluorescence with telomere fluorescence in situ hybridization) (left panel) and quantification (right panel) in p16 + cancer cells. (C-E) Sequential immunofluorescence quantifying p16 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells (CD45 + ), and the proliferative status (Ki67) of p16 + cells. (F) Senescence-associated β-galactosidase activity in human RBE CCA cells, proliferating versus doxorubicin or Gem/Cis-induced. (G) Single-nucleus RNA sequencing of human CCA (n=10 patients): UMAP of cell populations and expression of CDKN2A , CDKN1A , and MKI67 with senescence (SenMayo, Fridman, and Reactome cellular senescence) gene-set scores. (H) Multivariable Cox regression of senescence transcriptional scores and overall survival, adjusted for age, sex, and stage, performed independently in the Mayo discovery cohort (n=158, 108 events) and an external Asian cohort (OEP00105, n=244, 99 events). White arrows, senescent-like cancer cells. Scale bar, 50 µm. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Representative images (left panel) and quantification (right panel) of p16 + cancer cells in treatment-naive (n=17) and gemcitabine/cisplatin (Gem/Cis)-treated (n=22) human CCA. (B) Telomere-associated foci (γH2AX immunofluorescence with telomere fluorescence in situ hybridization) (left panel) and quantification (right panel) in p16 + cancer cells. (C-E) Sequential immunofluorescence quantifying p16 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells (CD45 + ), and the proliferative status (Ki67) of p16 + cells. (F) Senescence-associated β-galactosidase activity in human RBE CCA cells, proliferating versus doxorubicin or Gem/Cis-induced. (G) Single-nucleus RNA sequencing of human CCA (n=10 patients): UMAP of cell populations and expression of CDKN2A , CDKN1A , and MKI67 with senescence (SenMayo, Fridman, and Reactome cellular senescence) gene-set scores. (H) Multivariable Cox regression of senescence transcriptional scores and overall survival, adjusted for age, sex, and stage, performed independently in the Mayo discovery cohort (n=158, 108 events) and an external Asian cohort (OEP00105, n=244, 99 events). White arrows, senescent-like cancer cells. Scale bar, 50 µm. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques: Immunofluorescence, Fluorescence, In Situ Hybridization, Activity Assay, RNA Sequencing, Expressing

    (A) p21 + cancer cells in treatment-naive (n=15) versus Gem/Cis-treated (n=19) human CCA. (B-D) Sequential immunofluorescence of p21 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells (CD45 + ), and Ki67 status. (E-F) Senescence induction in SB1 cells with alisertib (0.2 µM, Aurora kinase A inhibitor) or etoposide (0.5 µM, topoisomerase II inhibitor) over 6 days, with drug withdrawn at day 4, confirmed by SA-β-gal and (E) p21 immunostaining (F).

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) p21 + cancer cells in treatment-naive (n=15) versus Gem/Cis-treated (n=19) human CCA. (B-D) Sequential immunofluorescence of p21 + cancer cells (KRT19 + ), cancer-associated fibroblasts (α-SMA + ), and immune cells (CD45 + ), and Ki67 status. (E-F) Senescence induction in SB1 cells with alisertib (0.2 µM, Aurora kinase A inhibitor) or etoposide (0.5 µM, topoisomerase II inhibitor) over 6 days, with drug withdrawn at day 4, confirmed by SA-β-gal and (E) p21 immunostaining (F).

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques: Immunofluorescence, Immunostaining

    (A) SB1 p21-ATTAC in vivo study schematic. (B) Representative gross images and tumor-burden quantification, vehicle versus AP-treated SB1 p21-ATTAC tumors. (C) Relative mRNA of p21-promoter tGFP reporter in veh and AP treated murine SB1 p21-ATTAC tumors. (D) p21 mean fluorescence intensity in veh and AP treated murine SB1 p21-ATTAC tumors. Scale bar, 100 µm. (E) Body weights of SB1 p16-ATTAC tumor bearing mice treated with or AP. (F) Monocytic myeloid-derived suppressor cell (M-MDSC), polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), regulatory T-cell (Treg), CD4 + T cells, and natural killer (NK) frequencies (% of CD45 + cells) in in SB1 p16-ATTAC tumors. (G) M1 macrophage, TAM, CD8 + CTL, granzyme B + CD8 + CTL frequencies in SB1 p21-ATTAC tumors. (H) Body weights of SB1 p21-ATTAC tumor bearing mice treated with or AP. (I) M-MDSC, PMN-MDSC, Treg, CD4 + T-cell, and NK-cell frequencies in SB1 p21-ATTAC tumors. Data are mean ± SEM; *, P<0.05; **, P<0.01.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) SB1 p21-ATTAC in vivo study schematic. (B) Representative gross images and tumor-burden quantification, vehicle versus AP-treated SB1 p21-ATTAC tumors. (C) Relative mRNA of p21-promoter tGFP reporter in veh and AP treated murine SB1 p21-ATTAC tumors. (D) p21 mean fluorescence intensity in veh and AP treated murine SB1 p21-ATTAC tumors. Scale bar, 100 µm. (E) Body weights of SB1 p16-ATTAC tumor bearing mice treated with or AP. (F) Monocytic myeloid-derived suppressor cell (M-MDSC), polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), regulatory T-cell (Treg), CD4 + T cells, and natural killer (NK) frequencies (% of CD45 + cells) in in SB1 p16-ATTAC tumors. (G) M1 macrophage, TAM, CD8 + CTL, granzyme B + CD8 + CTL frequencies in SB1 p21-ATTAC tumors. (H) Body weights of SB1 p21-ATTAC tumor bearing mice treated with or AP. (I) M-MDSC, PMN-MDSC, Treg, CD4 + T-cell, and NK-cell frequencies in SB1 p21-ATTAC tumors. Data are mean ± SEM; *, P<0.05; **, P<0.01.

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques: In Vivo, Fluorescence, Derivative Assay

    (A) Schema depicting in vivo study. SB tumor-bearing mice were treated for 2 weeks with vehicle, alisertib or etoposide. (B) Frequencies of M1 macrophages and M2-like tumor-associated macrophages (TAMs) (% of CD45 + cells) by flow cytometry. (C) Schematic of macrophage education; senescent-like state induced by alisertib or etoposide. (D) Bone-marrow-derived macrophage (BMDM) viability after conditioned medium from proliferating versus senescent-like cells. (E) Proliferation of CFSE-labeled CD8 + T cells co-cultured with educated BMDMs. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Schema depicting in vivo study. SB tumor-bearing mice were treated for 2 weeks with vehicle, alisertib or etoposide. (B) Frequencies of M1 macrophages and M2-like tumor-associated macrophages (TAMs) (% of CD45 + cells) by flow cytometry. (C) Schematic of macrophage education; senescent-like state induced by alisertib or etoposide. (D) Bone-marrow-derived macrophage (BMDM) viability after conditioned medium from proliferating versus senescent-like cells. (E) Proliferation of CFSE-labeled CD8 + T cells co-cultured with educated BMDMs. Data are mean ± SEM; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant.

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques: In Vivo, Flow Cytometry, Derivative Assay, Labeling, Cell Culture

    (A) M-MDSC, PMN-MDSC, and regulatory T-cell (Treg) frequencies (% of CD45 + cells) in the SB1 tumor bearing mice treated with vehicle, alisertib, or etoposide. (B) Schematic of subcutaneous tumor model study. (C) M1 macrophage, TAM, M-MDSC, PMN-MDSC, Treg frequencies in subcutaneous SB1 tumor bearing mice treated with vehicle, alisertib, or etoposide. Data are mean ± SEM; *, P<0.05; **; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) M-MDSC, PMN-MDSC, and regulatory T-cell (Treg) frequencies (% of CD45 + cells) in the SB1 tumor bearing mice treated with vehicle, alisertib, or etoposide. (B) Schematic of subcutaneous tumor model study. (C) M1 macrophage, TAM, M-MDSC, PMN-MDSC, Treg frequencies in subcutaneous SB1 tumor bearing mice treated with vehicle, alisertib, or etoposide. Data are mean ± SEM; *, P<0.05; **; ns, not significant.

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques:

    (A) Strategy for p16-promoter-restricted Gdf15 silencing (shRNA) and Gdf15 overexpression in orthotopic SB1 CCA. (B) Representative gross images (left) and tumor/liver weight quantification (right). (C-D) TAM and CD8 + CTL frequencies (% of CD45 + cells) in p16-shCtrl , SB-p16-shGdf15, and SB- p16-Gdf15 OE tumors via flow cytometry. (E) (A) EpCAM⁺GDF-15⁺ CCA cells (teal) adjacent to TAMs (pink); EpCAM⁺ (blue) and GDF-15⁺ (orange) transcripts shown. Scale bar 100 µm. (B) Per-TAM immunosuppressive score (per-sample z) versus distance to the nearest GDF-15⁺ or GDF-15⁻ CCA cell (TAMs ≤200 µm; n = 13,597 and 14,703 across three iCCA samples). Line/band: linear fit ± 95% CI; β and P from linear mixed-effects [score ∼ distance + (1|sample)]. The gradient is GDF-15⁺-specific (β = −3.79×10⁻³/µm, P < 10⁻¹⁰) and absent for GDF-15⁻ cells (β = +9.11×10⁻⁴/µm, P = 0.004). Scale bar, 100 µm. Data are mean ± SEM; *, P<0.05; ***, P<0.001; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Strategy for p16-promoter-restricted Gdf15 silencing (shRNA) and Gdf15 overexpression in orthotopic SB1 CCA. (B) Representative gross images (left) and tumor/liver weight quantification (right). (C-D) TAM and CD8 + CTL frequencies (% of CD45 + cells) in p16-shCtrl , SB-p16-shGdf15, and SB- p16-Gdf15 OE tumors via flow cytometry. (E) (A) EpCAM⁺GDF-15⁺ CCA cells (teal) adjacent to TAMs (pink); EpCAM⁺ (blue) and GDF-15⁺ (orange) transcripts shown. Scale bar 100 µm. (B) Per-TAM immunosuppressive score (per-sample z) versus distance to the nearest GDF-15⁺ or GDF-15⁻ CCA cell (TAMs ≤200 µm; n = 13,597 and 14,703 across three iCCA samples). Line/band: linear fit ± 95% CI; β and P from linear mixed-effects [score ∼ distance + (1|sample)]. The gradient is GDF-15⁺-specific (β = −3.79×10⁻³/µm, P < 10⁻¹⁰) and absent for GDF-15⁻ cells (β = +9.11×10⁻⁴/µm, P = 0.004). Scale bar, 100 µm. Data are mean ± SEM; *, P<0.05; ***, P<0.001; ns, not significant.

    Article Snippet: CD45 positive cells were enriched with CD45 (TIL) MicroBeads, mouse (Miltenyi # 130-110-618) according to the manufacturer’s instruction.

    Techniques: shRNA, Over Expression, Flow Cytometry